Thin Layer Chromatography Procedure

1. Add .1g TsCl, .037g 1,1 dimethyl ethanol, and 1mL of CH₂Cl₂ into 5mL vial.
2. Add .035g Pyrudine to the 5 ml vial.
3. Then put the triangular magnet in solution. Stir the solution for 30 mL by spinning the magnet.
4. Then you start TLC
5. Get small amount of TsCl and CH₂Cl₂ and mix in test tube.
6. Get a sheet and draw a think dashed line, with a pencil, about 1 cm from the bottom.
7. Then get to capillary tubes
8. Dip the first capillary tube in the mixture made in step 3. Then, make a small spot on the dashed line on the sheet.
9. Dip your second capillary tube on the TsCl and CH₂Cl₂ solution and make a small dot on the sheet. Make sure it’s separated from the first dot.
10. Then get a jar and add about ~20mL of Petroleum Ther in it. Put filter paper in bottom.
11. Then vertically put the sheet in the jar with the side with the dots closest to the Pyrudine. Close the jar when done.
12. Wait until the solvent travels almost all the way to the top, then remove the sheet from the jar.
13. Place the sheet under flourecent light and lightly draw what you see on the sheet with a pencil.
14. Measure the distance traveled by the mixture’s spot on the sheet and the distance traveled by the solvent (Pyrudine).
15. Then divide the distance traveled by the compound by the distance traveled by solvent to find the Rf value.