Tag: DNA sequencer

Watch, Do, Teach

by gwilson on Jun.07, 2012, under A Day in the Life...

Charlie Troxel, Li-Cor field engineer, gives pointers on interpretation of DNA sequencing gel analysis to Paul Pyenta and Sheena Banks.

Charlie Troxel, Li-Cor field engineer, gives pointers on interpretation of DNA sequencing gel analysis to Paul Pyenta and Sheena Banks.

One of the hallmarks of medical education is the concept of “watch, do, teach” – watch a procedure, do the procedure, teach the procedure – as a way to grow knowledge and skill in future doctors.  That same approach was used this week as Charlie Troxel from Li-Cor in Nebraska visited the BIMS labs to help McMurry faculty improve their skills with their DNA sequencer.  McMurry’s sequencer has been in service since 2008, but changes in personnel and projects necessitated some training upgrades.  McMurry faculty used PCR to amplify their target DNA and loaded the sequencer on Wednesday afternoon.  Fourteen hours later, the sequencing was done and teacher and students reconvened in the BIMS lab to observe the results.  Several software packages allowed the participants to evaluate the confidence of each base sequenced and provided ways to align sequences to test their consistency.  Pointers were given on how best to proceed with the plant samples Banks will analyze for Dr. Anna this summer as a part of a project to study genetic drift between specimens of a species collected in Old World and New World ecosystems.  The Li-Cor 4300 will get quite a workout between now and the end of the year.

That explains the “Watch” and “Do” activities.  The “Teach” component comes this fall as Banks puts the instrument to work teaching her Molecular Cell Biology Lab students how to do sequencing and analysis.  Nice.

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New Equipment

by gwilson on Jun.09, 2009, under A Day in the Life...

Hermle Z36We took delivery yesterday on a new high-speed centrifuge.  Our old one, purchased in 1996, put on quite a fireworks display a month or so ago and had to be put out to pasture.  The new one allows us to diversify our work a bit – swinging bucket rotor for 1 ml tubes, capacity for 250 ml bottles, etc.

The centrifuge is just one of several purchases to be made this summer – a Nanodrop spectrophotometer, another tissue culture hood, gel documentation system.  They follow a monumental year at McMurry in which we received a Li-COR GEMF Grant for a DNA sequencer, and purchased a variety of new equipment to outfit the molecular biology, genetics, and microbiology effort of the BIMS program.  As mentioned in an earlier post, we want our graduates to have experience with the equipment they will encounter in research, in medicine, in the workplace.  Hands-on experience teaches much better than theory on a chalkboard.

Our desire to impact “every student, every day” means these instruments will be used in a variety of classes by every student – not just a privileged few who go the extra mile in one-on-one research with faculty.  McMurry believes in the direction the BIMS program is headed and is willing to invest, even when times are tough.

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