BIMS

Tag: plant genetics

Watch, Do, Teach

by gwilson on Jun.07, 2012, under A Day in the Life...

Charlie Troxel, Li-Cor field engineer, gives pointers on interpretation of DNA sequencing gel analysis to Paul Pyenta and Sheena Banks.

Charlie Troxel, Li-Cor field engineer, gives pointers on interpretation of DNA sequencing gel analysis to Paul Pyenta and Sheena Banks.

One of the hallmarks of medical education is the concept of “watch, do, teach” – watch a procedure, do the procedure, teach the procedure – as a way to grow knowledge and skill in future doctors.  That same approach was used this week as Charlie Troxel from Li-Cor in Nebraska visited the BIMS labs to help McMurry faculty improve their skills with their DNA sequencer.  McMurry’s sequencer has been in service since 2008, but changes in personnel and projects necessitated some training upgrades.  McMurry faculty used PCR to amplify their target DNA and loaded the sequencer on Wednesday afternoon.  Fourteen hours later, the sequencing was done and teacher and students reconvened in the BIMS lab to observe the results.  Several software packages allowed the participants to evaluate the confidence of each base sequenced and provided ways to align sequences to test their consistency.  Pointers were given on how best to proceed with the plant samples Banks will analyze for Dr. Anna this summer as a part of a project to study genetic drift between specimens of a species collected in Old World and New World ecosystems.  The Li-Cor 4300 will get quite a workout between now and the end of the year.

That explains the “Watch” and “Do” activities.  The “Teach” component comes this fall as Banks puts the instrument to work teaching her Molecular Cell Biology Lab students how to do sequencing and analysis.  Nice.

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