Tag: yes assay
Every year the BIMS faculty sits down and discusses what research they might pursue for the sake of teaching and capstone projects. With the start of school only a week away, we have narrowed our focus to a couple of very promising avenues for student research. We thought you might like to know what’s made it to the top of our list for possible projects…
1. Bioactive compounds in the environment. Last year we began work to gear up use of the yeast estrogen screening (YES) assay to test soils and waters for the presence of estrogen-mimic compounds. Such compounds have been implicated in estrogen-fueled cancers, early onset of puberty, and other such health issues. Our early discussions this year have included involvement of Biochemistry in the use of the screen for testing area ground water and soils while Biology capstone students (BIMS 4201) may pursue use of biofermenters to produce mixed populations of microbes capable of destroying the chemicals. So, using our resources to identify the problem and find solutions. I like that!
2. Spore physiology and ecology. During my doctoral research, I made some discoveries that have gone unreported and have not been pursued since. My work was on Bacillus thuringiensis (Bt), a bacterium of economic importance because of the insecticidal crystal protein produced when it forms spores. Unlike the rest of the Bt world at the time, my interest was not in the crystal but in the biology of the spore. A part of that work involved studying, essentially, how diet influenced spore properties. I found those spores created in high sugar environments were larger and more resistant to heat, UV, and harsh chemicals, and germinated differently than did spores created in low sugar environments. I am teaching an Advanced Microbiology course (BIMS 4491) this fall where the students will resume the research with our goal to present results at the Texas Branch ASM meetings in March and to publish our results before the end of the year. Students leaving McMurry with presentations and publications is a good thing! Because the work is so expansive and offers so many opportunities for students to jump on-board, other students doing capstones may also find a piece of this puzzle they want to pursue. This research teaches some great basic biology and microbiology and has tremendous biomedical importance – after all, Bt is the simulant used for research on anthrax!
Some might look at the type of work our students pursue at McMurry and determine that the research done here is not as “cutting edge” and sophisticated as that done at large universities. Rightly so, and without apologies! Our intent is not to invite undergraduates to wash dishes or “piddle around” on the fringes of our research, but to be the main contributors to our work – much as graduate students are at those large universities. Every student is exposed to research here, and they are integral to our progress – not footnotes to graduate students’ success! Their work is the main course, the entree and not the parsley and onion soup. The fact of the matter is there are always plenty of questions of interest and importance to be answered that are left behind as the juggernaut of big science crashes forward. We will gladly fill in the blanks left behind as they rush onward. Such questions provide a fertile ground for learning and discovery. We are student-centered in our teaching and in our research. BIMS at McMurry is simply “science done better”.
We had another BUSY week in Biomedical Science courses.
- Freshman-level BIMS 1300 Intro to Scientific Research students learned how to use their Tablet PCs to gather data from a “Brain Test” all students took (determined analytical vs. creative, auditory vs. visual) and calculate standard error of the mean, as well as linear regression analysis of data sets. In the lab, students finished up their observation projects that will be presented in the coming week.
- The new microbiology course for allied health majors, BIOL 3403 Fundamentals of Microbiology participated in a webinar hosted by McMurry alumna Mary Lynn Smith (’83) on biofilms in healthcare. This was an example of how experts and professionals a thousand miles away can contribute to our students’ education.
- In BIOL 3410 Microbiology, students finished the identification of Gram positive bacteria found in their cars. They are working on research posters describing their studies and will turn those in next week. In short, they took samples from the HVAC and interior surfaces of their cars, isolated and purified bacteria, and pursued identifications of the Gram positive cocci found using conventional tests and the BD-BBL Crystal(TM) Rapid ID panels. Follow-up tests included testing for oxacillin-resistance, an indicator of community-borne MRSA.
- In BIMS 4391 Advanced Microbiology, students moved forward in their development of antibiotic-producing bacteria. They completed the identification of their endospore-formers using microscopy, conventional tests, and BD-BBL Crystal(TM) Rapid ID panels. Then, they grew their bacterium in batch culture, removed the cells and spores by centrifugation and filtration, and challenged six microbes (two Gram negative rods, two Gram positive cocci, two yeasts) with the filtrate in disk diffusion tests. Those antibiotic producers with the most promise will be grown in our new benchtop fermenters and their products characterized by chemical, physical, and physiological means to learn more.
- In our BIMS 4201 Capstone Research class, senior students began cultivating the Saccharomyces cerevisiae strain genetically-modified with human estrogen receptor as a prelude to the use of the YES assay for monitoring the presence of estrogen-mimics in the environment.
All this may sound way beyond the reach of normal college students in normal college classes. Not so! We find that students are more engaged in learning techniques and information when there’s a reason or goal – a pot of gold at the end of the rainbow! It is at the heart of the skills-laden, research-rich approach taken in teaching BIMS courses.
McMurry’s spring semester is underway and classes for Biomedical Science majors continue to draw interest from students and campus leaders. The BIMS 1300 Intro to Scientific Research course is filled beyond capacity. Taught by Dr. Wilson, students will explore what science is, how scientists work, and how the methods of science influence all of society. For instance, next week students will watch a video on the design firm IDEO and explore the basic science, applied science, engineering, and design that have gone into a variety of consumer products.
Dr. Benoit is teaching BIOL 1301 Unicellular Organisms to a healthy number of students. Their semester-long project will investigate protozoans and will culminate with identification, characterization, and photomicrography of single-celled organisms. This has proven to be a very popular and interesting class for new freshmen, and sets the stage well for a degree program filled with hands-on exploration of biomedical topics.
BIOL 3410 Microbiology is also filled to capacity and BIOL 3430 Human Physiology has a healthy enrollment. Both are part of the sophomore sequence for all BIMS majors. Dr. Wilson’s Micro course will feature lab projects looking at the microbial census of student cars, microbes in fresh foods, and viruses from the soil. As always, the focus is on learning knowledge and skills by jumping into research projects – students work as scientists to learn about microbiology. Dr. Sharp’s Human Phys will use a mixture of computer sims and hands-on biometrics to explore the workings of the human body.
Also being taught this semester is BIMS 4391 Advanced Microbiology. Dr. Wilson is leading five students on a quest to isolate and identify endospore-forming bacteria that produce antibiotics. Students will then produce the product using new benchtop fermenters and characterize the antibiotic product physically and chemically. The class is also considering a jaunt down to T-Bar-M ranch for the Spring Meeting of the Texas Branch of the American Society Microbiology, which emphasizes graduate and undergraduate research. ROAD TRIP!
Another unique feature of the BIMS program is the BIMS 4000 Junior Exam course, where students take a departmental diagnostic exam over their first two years of courses to help assess their learning to this point and to help the department assess the effectiveness of its courses in teaching fundamental information. The five students signed up for the course may take this online exam as often as needed to achieve a passing grade.
Finally, several students are engaged in capstone research this semester with Drs. Benoit and Wilson. They will be ramping up the YES assay for detecting estrogen-like compounds in environmental samples of water and soil. We’ve challenged them with developing the protocols for use on campus and developing the standard curve for the assay, then begin testing on some samples from area surface and ground waters.
So, it is a busy time for a healthy program. Bright students have chosen our unique approach to education and are thriving in the hands-on environment.